%0 Journal Article %A Herron, Lissa %A Pridans, Clare %A Turnbull, Matthew %A Smith, Nikki %A Lillico, Simon %A Sherman, Adrian %A Gilhooley, Hazel %A Wear, Martin %A Kurian, Dominic %A Papadakos, Grigorios %A Digard, Paul %A Hume, David %A Gill, Andrew %A Sang, Helen %D 2018 %T Additional file 5: of A chicken bioreactor for efficient production of functional cytokines %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_5_of_A_chicken_bioreactor_for_efficient_production_of_functional_cytokines/7532288 %R 10.6084/m9.figshare.7532288.v1 %2 https://springernature.figshare.com/ndownloader/files/14002976 %K Transgenic animals %K Biologics %K Recombinant proteins %K Cytokines %K Animal biotechnology %X Figure S5. pCSF1-Fc mono-dispersity, size and purity analysis. A) Size-exclusion chromatography multi-angled light scattering (SEC-MALS) was used to determine the molecular and mono-dispersity of pCSF1-Fc in solution. pCSF1-Fc eluted as a single peak (≥ 97% purity; As = 0.91) from a Superdex-200 Increase 10/300 GL size exclusion column with apparent molecular mass of ~ 110 ± 5 kDa and an Rs of 4.03 ± 0.4 nm (mean ± SD, n = 3). B) Elution profile of pCSF1-Fc from an RPC 5 4.6/150 ST column run. Chromatogram shows single sharp peak, with ≥98% purity. (DOCX 188 kb) %I figshare