%0 Journal Article %A Nguyen, James %A Fong, Jonathan %A Fong, Daniel %A Fong, Timothy %A Lucero, Rachael %A Gallimore, Jamie %A Burata, Olive %A Parungao, Kamille %A Rascón, Alberto %D 2018 %T Additional file 3: of Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Soluble_expression_of_recombinant_midgut_zymogen_native_propeptide_proteases_from_the_Aedes_aegypti_Mosquito_Utilizing_E_coli_as_a_host/7483904 %R 10.6084/m9.figshare.7483904.v1 %2 https://springernature.figshare.com/ndownloader/files/13866410 %K Aedes aegypti %K Midgut %K Proteases %K Zymogen %K Soluble expression %K Recombinant protein %K Escherichia coli %K Disulfide bond/bridge %X Figure S3. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale growth experiment of AaSPVII-NL non-induced SHuffle® E. coli cells (NEB) grown in TB media at 15 °C. Samples were collected at the given time points (in hours). The MW ladder is in kilo-Daltons (kDa). The gel shows both the total and soluble samples collected at the same time-points as in Fig. 4c There is no expression of AaSPVII-NL zymogen. In addition, very little to no BApNA activity is observed (plot on the right), similar to the pre-induction (0 h) and early post-induction (4, 8, 10 h) samples in Fig. 4c. (DOCX 746 kb) %I figshare