10.6084/m9.figshare.7483889.v1 James Nguyen James Nguyen Jonathan Fong Jonathan Fong Daniel Fong Daniel Fong Timothy Fong Timothy Fong Rachael Lucero Rachael Lucero Jamie Gallimore Jamie Gallimore Olive Burata Olive Burata Kamille Parungao Kamille Parungao Alberto Rascón Alberto Rascón Additional file 1: of Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host Springer Nature 2018 Aedes aegypti Midgut Proteases Zymogen Soluble expression Recombinant protein Escherichia coli Disulfide bond/bridge 2018-12-18 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Soluble_expression_of_recombinant_midgut_zymogen_native_propeptide_proteases_from_the_Aedes_aegypti_Mosquito_Utilizing_E_coli_as_a_host/7483889 Figure S1. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale growth experiment of AaET-NL non-induced SHuffle® E. coli cells (NEB) grown in TB media at 15 °C. Samples were collected at the given time points (in hours). The MW ladder is in kilo-Daltons (kDa). The gel shows both the total and soluble samples collected at the same time-points as in Fig. 4a There is no expression of AaET-NL zymogen. In addition, very little to no BApNA activity is observed (plot on the right), similar to the pre-induction (0 h) and early post-induction (3 h) sample in Fig. 4a. (DOCX 556 kb)