10.6084/m9.figshare.7483889.v1
James Nguyen
James
Nguyen
Jonathan Fong
Jonathan
Fong
Daniel Fong
Daniel
Fong
Timothy Fong
Timothy
Fong
Rachael Lucero
Rachael
Lucero
Jamie Gallimore
Jamie
Gallimore
Olive Burata
Olive
Burata
Kamille Parungao
Kamille
Parungao
Alberto Rascón
Alberto
Rascón
Additional file 1: of Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host
Springer Nature
2018
Aedes aegypti
Midgut
Proteases
Zymogen
Soluble expression
Recombinant protein
Escherichia coli
Disulfide bond/bridge
2018-12-18 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Soluble_expression_of_recombinant_midgut_zymogen_native_propeptide_proteases_from_the_Aedes_aegypti_Mosquito_Utilizing_E_coli_as_a_host/7483889
Figure S1. SDS-PAGE analysis and BApNA activity assays of samples collected from the small-scale growth experiment of AaET-NL non-induced SHuffle® E. coli cells (NEB) grown in TB media at 15 °C. Samples were collected at the given time points (in hours). The MW ladder is in kilo-Daltons (kDa). The gel shows both the total and soluble samples collected at the same time-points as in Fig. 4a There is no expression of AaET-NL zymogen. In addition, very little to no BApNA activity is observed (plot on the right), similar to the pre-induction (0 h) and early post-induction (3 h) sample in Fig. 4a. (DOCX 556 kb)