Additional file 3: of An evaluation of TAZ and YAP crosstalk with TGFβ signalling in canine osteosarcoma suggests involvement of hippo signalling in disease progression LuuAnita SchottCourtney JonesRobert PoonAndrew GoldingBrandon HamedRoa’a DeheshiBenjamin MutsaersAnthony WoodGeoffrey Viloria-PetitAlicia 2018 Figure S3. Representative immunoblots and densitometry demonstrating reduction in YAP protein levels post siRNA transfection at 24 hours. YAP levels were decreased with siRNA treatment by varying levels, as indicated by the percentages, when compared to the siRNA control (siCtrl), while TAZ levels were not affected. Experimental groups were normalized to loading control β-actin. Graphs depict the average fold change in TAZ or YAP expression relative to siCtrl ± SEM from three independent experiments. (PDF 15825 kb)