%0 Journal Article %A Takagi, Hiroshi %A Watanabe, Shunsuke %A Tanaka, Shoma %A Matsuura, Takakazu %A Mori, Izumi %A Hirayama, Takashi %A Shimada, Hiroshi %A Sakamoto, Atsushi %D 2018 %T Additional file 7: of Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_7_of_Disruption_of_ureide_degradation_affects_plant_growth_and_development_during_and_after_transition_from_vegetative_to_reproductive_stages/7369076 %R 10.6084/m9.figshare.7369076.v1 %2 https://springernature.figshare.com/ndownloader/files/13623452 %K Allantoate %K Allantoin %K Arabidopsis thaliana %K Catabolic pathway %K Nitrogen remobilization %K Phytohormone %K Purine base %K Ureide transport %X Figure S6. Characterization of ups1 and ups2 mutants. (a) Diagram of the T-DNA insertion in AtUPS1 in the ups1 mutant. Arrows indicate PCR primers; white boxes indicate untranslated regions; black boxes indicate exons; black lines indicate introns. (b) PCR-based genotyping of the ups1 mutant using primers specific to AtUPS1 (F1 and R1) and the left border sequence of the T-DNA (SAIL LB1). (c) Diagram of the T-DNA insertion in AtUPS2 in the ups2 mutant. Arrows indicate PCR primers; white boxes indicate untranslated regions; black boxes indicate exons; black lines indicate introns. (d) PCR-based genotyping of the ups1 mutant using primers specific to AtUPS2 (F2 and R2) and the left border sequence of the T-DNA (LBa1). (e) Semi-quantitative reverse transcription-PCR for estimating AtUPS1 and AtUPS2 mRNA levels in the mutant lines. VHA-A expression was simultaneously analyzed as an internal control. (PDF 865 kb) %I figshare