Skrypek, Nicolas Bruneel, Kenneth Vandewalle, Cindy Smedt, Eva Soen, Bieke Loret, Nele Taminau, Joachim Goossens, Steven Vandamme, Niels Berx, Geert MOESM2 of ZEB2 stably represses RAB25 expression through epigenetic regulation by SIRT1 and DNMTs during epithelial-to-mesenchymal transition Additional file 2: Figure S2. CDH1 and EpCAM are targeted by ZEB2 and SIRT1 modulating H3K9Ac level. (a) HA-ZEB2 ChIP assay after induction (+dox) analyzed on CDH1 and EpCAM promoter location using published sequences. (b) H3K9Ac ChIP assay performed in MDA-MB-231, 48 h after ZEB2 siRNA treatment (siZEB2). (c) RAB25 mRNA expression measured by qRT-PCR in HT29, MCF7, BT549 and MDA-MB-231. P values were determined using two-way ANOVA (***p < 0.001). (d) SIRT1 ChIP and (e) H3K9Ac ChIP assay performed after ZEB2 induction (+dox) with SIRT1 inhibitor (EX-527, 1 μM) (+dox/EX-527), in MCF7 analyzed on CDH1 and EpCAM promoter. Enrichments to input were calculated, control values were set as 1 and s.d. is shown. For all analyses, p values were determined using two-way ANOVA (*p < 0.05; **p < 0.01). NC = negative control. Three independent experiments were performed for all experiments. EMT;ZEB2;Epigenetic regulation;RAB25;SIRT1;DNMT 2018-11-16
    https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_ZEB2_stably_represses_RAB25_expression_through_epigenetic_regulation_by_SIRT1_and_DNMTs_during_epithelial-to-mesenchymal_transition/7355570
10.6084/m9.figshare.7355570.v1