%0 Journal Article %A Dawes, Brian %A Gao, Junling %A Atkins, Colm %A Nelson, Jacob %A Johnson, Kendra %A Wu, Ping %A Freiberg, Alexander %D 2018 %T Additional file 3: of Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Human_neural_stem_cell-derived_neuron_astrocyte_co-cultures_respond_to_La_Crosse_virus_infection_with_proinflammatory_cytokines_and_chemokines/7351151 %R 10.6084/m9.figshare.7351151.v1 %2 https://springernature.figshare.com/ndownloader/files/13583240 %K La Crosse virus %K Neural stem cells %K Neurons %K Astrocytes %K Inflammation %K Encephalitis %K Central nervous system %X Figure S3. Innate immune responses to inflammatory stimuli in neuron/astrocyte co-cultures. RT-PCR and BioPlex assays were performed to determine the cytokine and chemokine responses of neuron/astrocyte co-cultures. (a) Co-cultures were treated with either mock, Poly I:C, or heat inactivated LACV and at 48 HPI assessed for changes in gene expression for selected cytokines/chemokines via qRT-PCR. Values are reported as fold change relative to mock treatment normalized to 18S RNA. (b) Supernatant was collected and assayed for changes in selected cytokine/chemokine secretion via BioPlex assay. * P < 0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001. (PDF 207 kb) %I figshare