10.6084/m9.figshare.7238567.v1
Sumitra Shankar
Sumitra
Shankar
Ahalya Sreekumar
Ahalya
Sreekumar
Deepti Prasad
Deepti
Prasad
Ani Das
Ani
Das
M. Pillai
M.
Pillai
MOESM4 of Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design
Springer Nature
2018
Zinc-finger nucleases
TALEN
HPV
Cervical cancer
Gene editing
2018-10-22 05:00:00
Figure
https://springernature.figshare.com/articles/figure/MOESM4_of_Genome_editing_of_oncogenes_with_ZFNs_and_TALENs_caveats_in_nuclease_design/7238567
Additional file 4: Figure S4. TALEN designed against E6 could not yield editing in both SiHa and Caski cells. (A) Schematic of three TALEN binding sites on E6 gene. (B, C) TALEN targeting sequence at position 284 and its T7E1 assay in SiHa and CaSki cells showed no significant editing. (D, E) TALEN targeting sequence at position 57 and its T7E1 assay in SiHa and CaSki cells showed no significant editing. (F, G) TALEN targeting sequence at position 112 and its T7E1 assay in SiHa and CaSki cells showed no significant editing.