%0 Journal Article %A Lian, Yu %A Xiong, Fang %A Yang, Liting %A Bo, Hao %A Gong, Zhaojian %A Wang, Yumin %A Wei, Fang %A Tang, Yanyan %A Li, Xiayu %A Liao, Qianjin %A Wang, Hui %A Zhou, Ming %A Xiang, Bo %A Wu, Xu %A Li, Yong %A Li, Xiaoling %A Chen, Xiang %A Li, Guiyuan %A Guo, Can %A Zeng, Zhaoyang %A Xiong, Wei %D 2018 %T Additional file 2: of Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Long_noncoding_RNA_AFAP1-AS1_acts_as_a_competing_endogenous_RNA_of_miR-423-5p_to_facilitate_nasopharyngeal_carcinoma_metastasis_through_regulating_the_Rho_Rac_pathway/7216745 %R 10.6084/m9.figshare.7216745.v1 %2 https://springernature.figshare.com/ndownloader/files/13290149 %K Long noncoding RNA (lncRNA) %K AFAP1-AS1 %K miR-423-5p nasopharyngeal carcinoma (NPC) %K Competing endogenous RNA (ceRNA) %X Figure S1. Relative miR-423-5p expression levels as determined from GEO datasets. a. miR-423-5p was downregulated in NPC biopsies (T; n = 62) when compared with nontumor NPE tissues (N; n = 6) in the GSE73460 miRNA array, p = 0.0008. b. miR-423-5p expression was tightly associated with the TNM stage of NPC tumors in the GSE32906 miRNA array. N, n = 6; I-II, n = 4; III, n = 4; IV, n = 4; N vs IV, p = 0.02; N vs III, p = 0.04. c. Kaplan-Meier curve analysis of overall survival (OS) and relapse-free survival (RFS) of patients with low and high miR-423-5p expression using data from the GSE70970 dataset. HR, hazard ratio. Figure S2. Expression of some Rho/Rac proteins, such as RAB10, RAB11A, RAC2, PFN1, RHOA, RAC1, RHOC, LASP1 and RAB11B, was examined in 5-8F and HNE2 cells after overexpression of AFAP1-AS1. Figure S3. miR-423-5p could not target the 3′-UTR of RAC1. a. The schematic model showing the putative binding sites for miR-423-5p on 3′-untranslated regions (3′-UTR) of RAC1. b. Luciferase activities were measured in 5-8F and HNE2 cells cotransfected with miR-423-5p mimics, miR-423-5p inhibitors, or negative control, and the luciferase reporters containing the WT or MT RAC1 3′-UTR. Transfection of miR-423-5p mimics or inhibitors into 5-8F and HNE2 cells could not significantly reduce or increase the luciferase activity generated by the WT and the reporter vector containing the MT miR-423-5p binding site of the RAC1 3′-UTR reporter (pMIR-WT). Figure S4. Pearson correlation analysis was performed to evaluate the correlation between the expression of FOSL2 and AFAP1-AS1 using the GSE64634 dataset (r = 0.5256, p = 0.0365). Figure S5. A schematic model of AFAP1-AS1 competitively binding miR-423-5p to upregulate RAB11B or LASP1 or FOSL2 transcription factor signaling and accelerate NPC metastasis. (PDF 199 kb) %I figshare