Additional file 2: of Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway Yu Lian Fang Xiong Liting Yang Hao Bo Zhaojian Gong Yumin Wang Fang Wei Yanyan Tang Xiayu Li Qianjin Liao Hui Wang Ming Zhou Bo Xiang Xu Wu Yong Li Xiaoling Li Xiang Chen Guiyuan Li Can Guo Zhaoyang Zeng Wei Xiong 10.6084/m9.figshare.7216745.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Long_noncoding_RNA_AFAP1-AS1_acts_as_a_competing_endogenous_RNA_of_miR-423-5p_to_facilitate_nasopharyngeal_carcinoma_metastasis_through_regulating_the_Rho_Rac_pathway/7216745 Figure S1. Relative miR-423-5p expression levels as determined from GEO datasets. a. miR-423-5p was downregulated in NPC biopsies (T; n = 62) when compared with nontumor NPE tissues (N; n = 6) in the GSE73460 miRNA array, p = 0.0008. b. miR-423-5p expression was tightly associated with the TNM stage of NPC tumors in the GSE32906 miRNA array. N, n = 6; I-II, n = 4; III, n = 4; IV, n = 4; N vs IV, p = 0.02; N vs III, p = 0.04. c. Kaplan-Meier curve analysis of overall survival (OS) and relapse-free survival (RFS) of patients with low and high miR-423-5p expression using data from the GSE70970 dataset. HR, hazard ratio. Figure S2. Expression of some Rho/Rac proteins, such as RAB10, RAB11A, RAC2, PFN1, RHOA, RAC1, RHOC, LASP1 and RAB11B, was examined in 5-8F and HNE2 cells after overexpression of AFAP1-AS1. Figure S3. miR-423-5p could not target the 3′-UTR of RAC1. a. The schematic model showing the putative binding sites for miR-423-5p on 3′-untranslated regions (3′-UTR) of RAC1. b. Luciferase activities were measured in 5-8F and HNE2 cells cotransfected with miR-423-5p mimics, miR-423-5p inhibitors, or negative control, and the luciferase reporters containing the WT or MT RAC1 3′-UTR. Transfection of miR-423-5p mimics or inhibitors into 5-8F and HNE2 cells could not significantly reduce or increase the luciferase activity generated by the WT and the reporter vector containing the MT miR-423-5p binding site of the RAC1 3′-UTR reporter (pMIR-WT). Figure S4. Pearson correlation analysis was performed to evaluate the correlation between the expression of FOSL2 and AFAP1-AS1 using the GSE64634 dataset (r = 0.5256, p = 0.0365). Figure S5. A schematic model of AFAP1-AS1 competitively binding miR-423-5p to upregulate RAB11B or LASP1 or FOSL2 transcription factor signaling and accelerate NPC metastasis. (PDF 199 kb) 2018-10-16 05:00:00 Long noncoding RNA (lncRNA) AFAP1-AS1 miR-423-5p nasopharyngeal carcinoma (NPC) Competing endogenous RNA (ceRNA)