10.6084/m9.figshare.7216229.v1
Xudong Zhu
Xudong
Zhu
Xiang Shi
Xiang
Shi
Ju Chu
Ju
Chu
Bangce Ye
Bangce
Ye
Peng Zuo
Peng
Zuo
Yonghong Wang
Yonghong
Wang
MOESM1 of Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology
Springer Nature
2018
Bacillus coagulans
Microdroplets
Morphological heterogeneity
Single cell
2018-10-16 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM1_of_Quantitative_analysis_of_the_growth_of_individual_Bacillus_coagulans_cells_by_microdroplet_technology/7216229
Additional file 1: Fig. S1. The method of pixels counting. (A) Original photo of the droplets. (B) Adjusted image by adjusting the “threshold” and the pixels were automatically counted. Fig. S2. The growth curve of Bacillus coagulans in shake flask without (black) and with pHrodo (red). The data were obtained from three parallel experiments for each. Fig. S3. Lactic acid assay in microdroplets. (A) Fluorescence imaging of microdroplets containing broth with a range of lactic acid, 8 g/L (I), 11 g/L (II), 12 g/L (III), 14 g/L (IV). (B) The corresponding average gray value derive from the fluorescence intensity of the microdroplets in the images of Fig. S1 A.