%0 Figure %A Jiang, Pingping %A Zhang, Ke %A Ding, Zhaohua %A He, Qiuxia %A Li, Wendi %A Zhu, Shuangfeng %A Cheng, Wen %A Zhang, Kewei %A Li, Kunpeng %D 2018 %T Additional file 5: of Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding %U https://springernature.figshare.com/articles/figure/Additional_file_5_of_Characterization_of_a_strong_and_constitutive_promoter_from_the_Arabidopsis_serine_carboxypeptidase-like_gene_AtSCPL30_as_a_potential_tool_for_crop_transgenic_breeding/7120481 %R 10.6084/m9.figshare.7120481.v1 %2 https://springernature.figshare.com/ndownloader/files/13102079 %K AtSCPL30 %K CaMV35S %K Constitutive promoter %K GUS analysis %K Nicotiana benthamiana %K Stress treatment %X Figure S4. GUS staining of detached leaves of Nicotiana benthamiana transgenic plants under normal and salt-stress conditions. Ninety leaf discs (diameter 0.5 cm) from fifteen 60-day-old individual plants (5 individual plants/ line, 3 lines for each construct) of PD1-PD9 and CaMV35S transgenic plants were incubated in liquid 1/2 MS medium supplemented with 200 mM NaCl at 25 °C for 3, 6, 12, 24, 48, and 72 h; leaf discs floated in liquid 1/2 MS medium were used as control. The leaf discs of PD1-PD9 and CaMV35S transgenic plants were then incubated in staining solution at 37 °C for 3 h. Finally, the samples were observed and photographed after decolorization. (JPG 3572 kb) %I figshare