10.6084/m9.figshare.7120151.v1
Zhongyuan Tan
Zhongyuan
Tan
Wanpo Zhang
Wanpo
Zhang
Jianhong Sun
Jianhong
Sun
Zuquan Fu
Zuquan
Fu
Xianliang Ke
Xianliang
Ke
Caishang Zheng
Caishang
Zheng
Yuan Zhang
Yuan
Zhang
Penghui Li
Penghui
Li
Yan Liu
Yan
Liu
Qinxue Hu
Qinxue
Hu
Hanzhong Wang
Hanzhong
Wang
Zhenhua Zheng
Zhenhua
Zheng
Additional file 2: of ZIKV infection activates the IRE1-XBP1 and ATF6 pathways of unfolded protein response in neural cells
Springer Nature
2018
Zika virus
Neural cell
Neuropathogenesis
ER stress
Unfolded protein response
2018-09-21 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_2_of_ZIKV_infection_activates_the_IRE1-XBP1_and_ATF6_pathways_of_unfolded_protein_response_in_neural_cells/7120151
The model of ZIKV infection in human neural cells, CCF-STTG1, and SK-N-SH cell lines. a, b CCF-STTG1 and SK-N-SH were infected with ZIKV at an MOI of 5 PFU/cell. RNA was extracted and first strand cDNA was synthesized. A standard curve of RT-qPCR was built and the absolute quantification of the viral nucleic acid was calculated respectively. Data represented three independent experiments and error bars indicate mean ± SD. c, d Detection of viral envelope protein at 24 hpi by confocal immunofluorescence microscopy. The nucleus was visualized using Hoechst 33258 (blue), and the envelope protein was stained with the anti-ZIKV envelope protein antibody followed by Goat anti-Mouse IgG secondary antibody, Texas Red®-X (red). All the images were captured at × 10 magnification. One out of three independent experiments is shown, (c) CCF-STTG1 and (d) SK-N-SH. (EPS 1006 kb)