10.6084/m9.figshare.7120151.v1 Zhongyuan Tan Zhongyuan Tan Wanpo Zhang Wanpo Zhang Jianhong Sun Jianhong Sun Zuquan Fu Zuquan Fu Xianliang Ke Xianliang Ke Caishang Zheng Caishang Zheng Yuan Zhang Yuan Zhang Penghui Li Penghui Li Yan Liu Yan Liu Qinxue Hu Qinxue Hu Hanzhong Wang Hanzhong Wang Zhenhua Zheng Zhenhua Zheng Additional file 2: of ZIKV infection activates the IRE1-XBP1 and ATF6 pathways of unfolded protein response in neural cells Springer Nature 2018 Zika virus Neural cell Neuropathogenesis ER stress Unfolded protein response 2018-09-21 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_of_ZIKV_infection_activates_the_IRE1-XBP1_and_ATF6_pathways_of_unfolded_protein_response_in_neural_cells/7120151 The model of ZIKV infection in human neural cells, CCF-STTG1, and SK-N-SH cell lines. a, b CCF-STTG1 and SK-N-SH were infected with ZIKV at an MOI of 5 PFU/cell. RNA was extracted and first strand cDNA was synthesized. A standard curve of RT-qPCR was built and the absolute quantification of the viral nucleic acid was calculated respectively. Data represented three independent experiments and error bars indicate mean ± SD. c, d Detection of viral envelope protein at 24 hpi by confocal immunofluorescence microscopy. The nucleus was visualized using Hoechst 33258 (blue), and the envelope protein was stained with the anti-ZIKV envelope protein antibody followed by Goat anti-Mouse IgG secondary antibody, Texas Red®-X (red). All the images were captured at × 10 magnification. One out of three independent experiments is shown, (c) CCF-STTG1 and (d) SK-N-SH. (EPS 1006 kb)