Additional file 10: of Renal cancer: new models and approach for personalizing therapy di MartinoSimona De LucaGabriele GrassiLudovica FedericiGiulia AlfonsiRomina SignoreMichele AddarioAntonio De SalvoLaura FrancescangeliFederica SanchezMassimo TirelliValentina MutoGiovanni SperdutiIsabella SentinelliSteno CostantiniManuela PasquiniLuca MilellaMichele HaouiMustapha SimoneGiuseppe GallucciMichele MariaRuggero De BonciDésirée 2018 Figure S7. (A) Two-way unsupervised hierarchical clustering of RPPA data of 2 G1, 7 G2, 8 G3, and 3 G4 ccRCC populations for the expression of stem cell markers reported as heatmap. (B) mRNA expression of SOX2 gene in G2, G3, and G4 ccRCC samples as assessed by RT-qPCR. Mean of three independent experiments is reported. RRN18S was used as endogenous control. *p < 0.05 (C) Representative immunofluorescence staining of tumor derived spheroids showing positivity for SOX2 (red). Merge is the over-lapping of SOX2 and DAPI staining. Confocal-microscope used Olympus, Fluoview FV1000 (Tokyo, Japan, http://www.olympus-global.com), magnification 40X. (PDF 246 kb)