Additional file 10: of Renal cancer: new models and approach for personalizing therapy
Simona di Martino
Gabriele De Luca
Ludovica Grassi
Giulia Federici
Romina Alfonsi
Michele Signore
Antonio Addario
Laura De Salvo
Federica Francescangeli
Massimo Sanchez
Valentina Tirelli
Giovanni Muto
Isabella Sperduti
Steno Sentinelli
Manuela Costantini
Luca Pasquini
Michele Milella
Mustapha Haoui
Giuseppe Simone
Michele Gallucci
Ruggero De Maria
Désirée Bonci
10.6084/m9.figshare.7052711.v1
https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_of_Renal_cancer_new_models_and_approach_for_personalizing_therapy/7052711
Figure S7. (A) Two-way unsupervised hierarchical clustering of RPPA data of 2 G1, 7 G2, 8 G3, and 3 G4 ccRCC populations for the expression of stem cell markers reported as heatmap. (B) mRNA expression of SOX2 gene in G2, G3, and G4 ccRCC samples as assessed by RT-qPCR. Mean of three independent experiments is reported. RRN18S was used as endogenous control. *p < 0.05 (C) Representative immunofluorescence staining of tumor derived spheroids showing positivity for SOX2 (red). Merge is the over-lapping of SOX2 and DAPI staining. Confocal-microscope used Olympus, Fluoview FV1000 (Tokyo, Japan, http://www.olympus-global.com), magnification 40X. (PDF 246 kb)
2018-09-05 05:00:00
Renal cell carcinoma
Patient-derived xenografts
Reverse phase protein array; personalized therapy
Targeted therapy