Additional file 10: of Renal cancer: new models and approach for personalizing therapy Simona di Martino Gabriele De Luca Ludovica Grassi Giulia Federici Romina Alfonsi Michele Signore Antonio Addario Laura De Salvo Federica Francescangeli Massimo Sanchez Valentina Tirelli Giovanni Muto Isabella Sperduti Steno Sentinelli Manuela Costantini Luca Pasquini Michele Milella Mustapha Haoui Giuseppe Simone Michele Gallucci Ruggero De Maria Désirée Bonci 10.6084/m9.figshare.7052711.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_10_of_Renal_cancer_new_models_and_approach_for_personalizing_therapy/7052711 Figure S7. (A) Two-way unsupervised hierarchical clustering of RPPA data of 2 G1, 7 G2, 8 G3, and 3 G4 ccRCC populations for the expression of stem cell markers reported as heatmap. (B) mRNA expression of SOX2 gene in G2, G3, and G4 ccRCC samples as assessed by RT-qPCR. Mean of three independent experiments is reported. RRN18S was used as endogenous control. *p < 0.05 (C) Representative immunofluorescence staining of tumor derived spheroids showing positivity for SOX2 (red). Merge is the over-lapping of SOX2 and DAPI staining. Confocal-microscope used Olympus, Fluoview FV1000 (Tokyo, Japan, http://www.olympus-global.com), magnification 40X. (PDF 246 kb) 2018-09-05 05:00:00 Renal cell carcinoma Patient-derived xenografts Reverse phase protein array; personalized therapy Targeted therapy