%0 Journal Article %A Rincón, Esther %A Rocha-Gregg, Briana %A Collins, Sean %D 2018 %T Additional file 1: of A map of gene expression in neutrophil-like cell lines %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_A_map_of_gene_expression_in_neutrophil-like_cell_lines/6891392 %R 10.6084/m9.figshare.6891392.v1 %2 https://springernature.figshare.com/ndownloader/files/12566078 %K Neutrophil-like cell line %K Differentiation protocol %K Chemotaxis %K RNA-seq %K Database %K Neutrophil %X Comparison of cell surface marker expression with different differentiation protocols. PLB-985 cells were differentiated into a neutrophil-like state by culturing in media supplemented with 1.3% DMSO and 2 μM ATRA for 4 days, 1.3% DMSO and 2 μM ATRA for 6 days, 0.5% DMF for 6 days, 1 μM ATRA for 6 days, 2 μM ATRA for 6 days, or 0.5% DMF and 2 μM ATRA for 6 days. Undifferentiated cells were also analyzed. Cells were stained with an antibody against CD11b, chosen as an early differentiation marker (a), or with FLPEP (a fluorescent ligand of FPR1), as a late differentiation marker (b). (c) PLB-985 cells differentiated for 6 days with either 1.3% DMSO, 1.3% DMSO and 2% Nutridoma-CS, 0.5% DMF and 2 μM ATRA, or dbcAMP were mixed in suspension with pHrodo Green-labeled dead Staphylococcus aureus bioparticles for 2 hours at 37 degrees. Phagocytosis of the particles was then analyzed by cytometry and data was analyzed using MATLAB. (PDF 162 kb) %I figshare