Additional file 5: of Consistent and reproducible long-term in vitro growth of health and disease-associated oral subgingival biofilms Irina Velsko Luciana Shaddox 10.6084/m9.figshare.6808253.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_5_of_Consistent_and_reproducible_long-term_in_vitro_growth_of_health_and_disease-associated_oral_subgingival_biofilms/6808253 Figure S3. Alpha diversity of healthy and disease site-derived biofilms throughout the generations. (a) Chao1 index rarefaction curves at generations 1, 4, 7, and 9. (b) Chao1 index of biofilms by generation at 20000 reads. (c) Shannon index rarefaction curves at generations 1, 4, 7, and 9. (d) Shannon index in biofilms by generation at 20000 reads. Nā€‰=ā€‰2 biofilms per group so statistical significance could not be calculated. Box plots show 5ā€“95 percentile. All error bars indicate Ā± SD. (PDF 103 kb) 2018-07-11 05:00:00 Biofilm growth Dental plaque Subgingival plaque Oral microbiome Periodontal disease In vitro model Model system