10.6084/m9.figshare.6616175.v1
Jingjing Xu
Jingjing
Xu
Jun Zhou
Jun
Zhou
Hanjue Dai
Hanjue
Dai
Fei Liu
Fei
Liu
Wenjing Li
Wenjing
Li
Wenjuan Wang
Wenjuan
Wang
Feng Guo
Feng
Guo
MOESM3 of CHIP functions as an oncogene by promoting colorectal cancer metastasis via activation of MAPK and AKT signaling and suppression of E-cadherin
Springer Nature
2018
CHIP
Colorectal cancer
Oncogene
Metastasis
Epithelial–mesenchymal transition
2018-06-19 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM3_of_CHIP_functions_as_an_oncogene_by_promoting_colorectal_cancer_metastasis_via_activation_of_MAPK_and_AKT_signaling_and_suppression_of_E-cadherin/6616175
Additional file 3. Establishment of CHIP-sliencing and CHIP-overexpressing DLD-1 cells. (a) qRT-PCR analysis of the mRNA expression of CHIP between the two established siCHIP and sictrl cell lines. β-actin normalized gene expression, measured in triplicates was displayed. Significant differences were indicated (Student’s t-test, ***P < 0.001). (b) The protein expression of CHIP in the whole cell extract of siCHIP and sictrl cell lines was analyzed by western blotting. The level of each protein was normalized against Actin. (c) qRT-PCR analysis of the mRNA expression of CHIP between the two established hCHIP and ctrl cells. β-actin normalized gene expression, measured in triplicates is displayed. Significant differences were indicated (Student’s t-test, ***P < 0.001). (d) The protein expression of CHIP in the whole cell extract of hCHIP and ctrl cells was analyzed by western blotting. The level of each protein was normalized against Actin.