MOESM7 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies Itziar Ubillos Alfons Jiménez Marta Vidal Paul Bowyer Deepak Gaur Sheetij Dutta Benoit Gamain Ross Coppel Virander Chauhan David Lanar Chetan Chitnis Evelina Angov James Beeson David Cavanagh Joseph Campo Ruth Aguilar Carlota Dobaño 10.6084/m9.figshare.6407306.v1 https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407306 Additional file 7. Levels of IgM and IgE measured to the 40-multiplex panel in the WHO reference reagent and IgM pool compared to negative control and blanks under two different incubation conditions. Incubation conditions compared are: 4 °C (4 °C ON) vs 2 h at 37 °C (37 °C 2 h). A) Predicted 5PL curves of IgM levels in the IgM pool. B) Predicted 5PL curves of IgM levels in the WHO reference reagent. C) IgE levels in the WHO reference reagent. 2018-06-01 05:00:00 Plasmodium falciparum Quantitative suspension array technology Multiplex IgG, IgG1, IgG2, IgG3, IgG4 subclasses IgM IgE Reference reagent Incubation conditions Assay performance