MOESM7 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies
Itziar Ubillos
Alfons Jiménez
Marta Vidal
Paul Bowyer
Deepak Gaur
Sheetij Dutta
Benoit Gamain
Ross Coppel
Virander Chauhan
David Lanar
Chetan Chitnis
Evelina Angov
James Beeson
David Cavanagh
Joseph Campo
Ruth Aguilar
Carlota Dobaño
10.6084/m9.figshare.6407306.v1
https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407306
Additional file 7. Levels of IgM and IgE measured to the 40-multiplex panel in the WHO reference reagent and IgM pool compared to negative control and blanks under two different incubation conditions. Incubation conditions compared are: 4 °C (4 °C ON) vs 2 h at 37 °C (37 °C 2 h). A) Predicted 5PL curves of IgM levels in the IgM pool. B) Predicted 5PL curves of IgM levels in the WHO reference reagent. C) IgE levels in the WHO reference reagent.
2018-06-01 05:00:00
Plasmodium falciparum
Quantitative suspension array technology
Multiplex
IgG, IgG1, IgG2, IgG3, IgG4 subclasses
IgM
IgE
Reference reagent
Incubation conditions
Assay performance