10.6084/m9.figshare.6407306.v1 Itziar Ubillos Itziar Ubillos Alfons Jiménez Alfons Jiménez Marta Vidal Marta Vidal Paul Bowyer Paul Bowyer Deepak Gaur Deepak Gaur Sheetij Dutta Sheetij Dutta Benoit Gamain Benoit Gamain Ross Coppel Ross Coppel Virander Chauhan Virander Chauhan David Lanar David Lanar Chetan Chitnis Chetan Chitnis Evelina Angov Evelina Angov James Beeson James Beeson David Cavanagh David Cavanagh Joseph Campo Joseph Campo Ruth Aguilar Ruth Aguilar Carlota Dobaño Carlota Dobaño MOESM7 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies Springer Nature 2018 Plasmodium falciparum Quantitative suspension array technology Multiplex IgG, IgG1, IgG2, IgG3, IgG4 subclasses IgM IgE Reference reagent Incubation conditions Assay performance 2018-06-01 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407306 Additional file 7. Levels of IgM and IgE measured to the 40-multiplex panel in the WHO reference reagent and IgM pool compared to negative control and blanks under two different incubation conditions. Incubation conditions compared are: 4 °C (4 °C ON) vs 2 h at 37 °C (37 °C 2 h). A) Predicted 5PL curves of IgM levels in the IgM pool. B) Predicted 5PL curves of IgM levels in the WHO reference reagent. C) IgE levels in the WHO reference reagent.