MOESM6 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies
Itziar Ubillos
Alfons Jiménez
Marta Vidal
Paul Bowyer
Deepak Gaur
Sheetij Dutta
Benoit Gamain
Ross Coppel
Virander Chauhan
David Lanar
Chetan Chitnis
Evelina Angov
James Beeson
David Cavanagh
Joseph Campo
Ruth Aguilar
Carlota Dobaño
10.6084/m9.figshare.6407303.v1
https://springernature.figshare.com/articles/journal_contribution/MOESM6_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407303
Additional file 6. Levels of IgG and IgG2-4 to 15 antigens measured in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific antibody levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h (37 °C 2 h), 4 °C overnight (4 °C ON) and room temperature for 1 h (RT 1 h). “neg” means negative control.
2018-06-01 05:00:00
Plasmodium falciparum
Quantitative suspension array technology
Multiplex
IgG, IgG1, IgG2, IgG3, IgG4 subclasses
IgM
IgE
Reference reagent
Incubation conditions
Assay performance