MOESM6 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies Itziar Ubillos Alfons Jiménez Marta Vidal Paul Bowyer Deepak Gaur Sheetij Dutta Benoit Gamain Ross Coppel Virander Chauhan David Lanar Chetan Chitnis Evelina Angov James Beeson David Cavanagh Joseph Campo Ruth Aguilar Carlota Dobaño 10.6084/m9.figshare.6407303.v1 https://springernature.figshare.com/articles/journal_contribution/MOESM6_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407303 Additional file 6. Levels of IgG and IgG2-4 to 15 antigens measured in the WHO reference reagent compared to negative control and blanks under three different incubation conditions. Curve plots of the antigen-specific antibody levels measured in serial dilutions of the WHO reference reagent, negative control and blanks at three different incubation conditions: 37 °C 2 h (37 °C 2 h), 4 °C overnight (4 °C ON) and room temperature for 1 h (RT 1 h). “neg” means negative control. 2018-06-01 05:00:00 Plasmodium falciparum Quantitative suspension array technology Multiplex IgG, IgG1, IgG2, IgG3, IgG4 subclasses IgM IgE Reference reagent Incubation conditions Assay performance