%0 Journal Article %A Ubillos, Itziar %A Jiménez, Alfons %A Vidal, Marta %A Bowyer, Paul %A Gaur, Deepak %A Dutta, Sheetij %A Gamain, Benoit %A Coppel, Ross %A Chauhan, Virander %A Lanar, David %A Chitnis, Chetan %A Angov, Evelina %A Beeson, James %A Cavanagh, David %A Campo, Joseph %A Aguilar, Ruth %A Dobaño, Carlota %D 2018 %T MOESM2 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies %U https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407255 %R 10.6084/m9.figshare.6407255.v1 %2 https://springernature.figshare.com/ndownloader/files/11800199 %K Plasmodium falciparum %K Quantitative suspension array technology %K Multiplex %K IgG, IgG1, IgG2, IgG3, IgG4 subclasses %K IgM %K IgE %K Reference reagent %K Incubation conditions %K Assay performance %X Additional file 2. Comparison of the WHO reference reagent, IgM pool and RTS,S samples responses to the 40-antigen multiplex panel incubating at 4 °C ON. IgG, IgG1–4 subclasses, IgM and IgE were measured in the respective pools and samples. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG1–4; and 1:2 starting at 1:10 for IgE). Data on IgG3 and IgE levels measured in RTS,S vaccinees were not available. Isolated dots represent the levels measured in the technical blanks. %I figshare