10.6084/m9.figshare.6407255.v1 Itziar Ubillos Itziar Ubillos Alfons Jiménez Alfons Jiménez Marta Vidal Marta Vidal Paul Bowyer Paul Bowyer Deepak Gaur Deepak Gaur Sheetij Dutta Sheetij Dutta Benoit Gamain Benoit Gamain Ross Coppel Ross Coppel Virander Chauhan Virander Chauhan David Lanar David Lanar Chetan Chitnis Chetan Chitnis Evelina Angov Evelina Angov James Beeson James Beeson David Cavanagh David Cavanagh Joseph Campo Joseph Campo Ruth Aguilar Ruth Aguilar Carlota Dobaño Carlota Dobaño MOESM2 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies Springer Nature 2018 Plasmodium falciparum Quantitative suspension array technology Multiplex IgG, IgG1, IgG2, IgG3, IgG4 subclasses IgM IgE Reference reagent Incubation conditions Assay performance 2018-06-01 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407255 Additional file 2. Comparison of the WHO reference reagent, IgM pool and RTS,S samples responses to the 40-antigen multiplex panel incubating at 4 °C ON. IgG, IgG1–4 subclasses, IgM and IgE were measured in the respective pools and samples. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG1–4; and 1:2 starting at 1:10 for IgE). Data on IgG3 and IgE levels measured in RTS,S vaccinees were not available. Isolated dots represent the levels measured in the technical blanks.