10.6084/m9.figshare.6407255.v1
Itziar Ubillos
Itziar
Ubillos
Alfons Jiménez
Alfons
Jiménez
Marta Vidal
Marta
Vidal
Paul Bowyer
Paul
Bowyer
Deepak Gaur
Deepak
Gaur
Sheetij Dutta
Sheetij
Dutta
Benoit Gamain
Benoit
Gamain
Ross Coppel
Ross
Coppel
Virander Chauhan
Virander
Chauhan
David Lanar
David
Lanar
Chetan Chitnis
Chetan
Chitnis
Evelina Angov
Evelina
Angov
James Beeson
James
Beeson
David Cavanagh
David
Cavanagh
Joseph Campo
Joseph
Campo
Ruth Aguilar
Ruth
Aguilar
Carlota Dobaño
Carlota
Dobaño
MOESM2 of Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1–4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies
Springer Nature
2018
Plasmodium falciparum
Quantitative suspension array technology
Multiplex
IgG, IgG1, IgG2, IgG3, IgG4 subclasses
IgM
IgE
Reference reagent
Incubation conditions
Assay performance
2018-06-01 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM2_of_Optimization_of_incubation_conditions_of_Plasmodium_falciparum_antibody_multiplex_assays_to_measure_IgG_IgG1_4_IgM_and_IgE_using_standard_and_customized_reference_pools_for_sero-epidemiological_and_vaccine_studies/6407255
Additional file 2. Comparison of the WHO reference reagent, IgM pool and RTS,S samples responses to the 40-antigen multiplex panel incubating at 4 °C ON. IgG, IgG1–4 subclasses, IgM and IgE were measured in the respective pools and samples. The plots represent the levels of antibodies measured in serial dilutions of the positive pools (1:3 starting at 1:50 for IgG, IgG1–4 and IgM; and 1:2 starting at 1:10 for IgE), and the RTS,S samples (1:10 starting at 1:500 for IgG, 1:100 for IgM, 1:50 for IgG1–4; and 1:2 starting at 1:10 for IgE). Data on IgG3 and IgE levels measured in RTS,S vaccinees were not available. Isolated dots represent the levels measured in the technical blanks.