MOESM2 of Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask
P. Philip
D. Kern
J. Goldmanns
F. Seiler
A. Schulte
T. Habicher
J. BĂźchs
10.6084/m9.figshare.6243809.v1
https://springernature.figshare.com/articles/figure/MOESM2_of_Parallel_substrate_supply_and_pH_stabilization_for_optimal_screening_of_E_coli_with_the_membrane-based_fed-batch_shake_flask/6243809
Additional file 2. Depiction of glucose amounts in the reservoir over the cultivation time. Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, blue dextran concentration: 1 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm2; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP.
2018-05-09 05:00:00
Fed-batch
Shake flask
pH
Osmolality
MOPS buffer
Secondary substrate limitation
Screening
RAMOS
Escherichia coli