MOESM2 of Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask P. Philip D. Kern J. Goldmanns F. Seiler A. Schulte T. Habicher J. Bßchs 10.6084/m9.figshare.6243809.v1 https://springernature.figshare.com/articles/figure/MOESM2_of_Parallel_substrate_supply_and_pH_stabilization_for_optimal_screening_of_E_coli_with_the_membrane-based_fed-batch_shake_flask/6243809 Additional file 2. Depiction of glucose amounts in the reservoir over the cultivation time. Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, blue dextran concentration: 1 g/L, temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm2; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP. 2018-05-09 05:00:00 Fed-batch Shake flask pH Osmolality MOPS buffer Secondary substrate limitation Screening RAMOS Escherichia coli