10.6084/m9.figshare.6193850.v1
Virginie Maillard
Virginie
Maillard
Alice Desmarchais
Alice
Desmarchais
Maeva Durcin
Maeva
Durcin
Svetlana Uzbekova
Svetlana
Uzbekova
Sebastien Elis
Sebastien
Elis
Additional file 4: of Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells
Springer Nature
2018
Bovine
Gene expression
Lipid
N-3 PUFA
DHA
Folliculogenesis
MAPK
AKT
AMPK
Signaling pathways
Free fatty acid receptor 4 (FFAR4)
2018-04-26 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_4_of_Docosahexaenoic_acid_DHA_effects_on_proliferation_and_steroidogenesis_of_bovine_granulosa_cells/6193850
Figure S3. Gene expression of (A) free fatty acid receptor 1 (FFAR1) and (B) free fatty acid receptor 4 (FFAR4) in bovine ovarian cells. Total mRNA was extracted from the ovarian cortex (CX), thecal cells (TH), granulosa cells (GC) and cumulus cells (CC). Total mRNA was then reverse-transcribed and real-time RT-PCR was performed. The geometric mean of two housekeeping genes (RPL19- ribosomal protein L19 and RPS9- ribosomal protein S9) was used to normalize gene expression. Results of 2 to 4 independent samples are presented as means Âą SEM. Bars with different superscripts are significantly different (pâ<â0.05). (TIF 59 kb)