10.6084/m9.figshare.6193850.v1 Virginie Maillard Virginie Maillard Alice Desmarchais Alice Desmarchais Maeva Durcin Maeva Durcin Svetlana Uzbekova Svetlana Uzbekova Sebastien Elis Sebastien Elis Additional file 4: of Docosahexaenoic acid (DHA) effects on proliferation and steroidogenesis of bovine granulosa cells Springer Nature 2018 Bovine Gene expression Lipid N-3 PUFA DHA Folliculogenesis MAPK AKT AMPK Signaling pathways Free fatty acid receptor 4 (FFAR4) 2018-04-26 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_4_of_Docosahexaenoic_acid_DHA_effects_on_proliferation_and_steroidogenesis_of_bovine_granulosa_cells/6193850 Figure S3. Gene expression of (A) free fatty acid receptor 1 (FFAR1) and (B) free fatty acid receptor 4 (FFAR4) in bovine ovarian cells. Total mRNA was extracted from the ovarian cortex (CX), thecal cells (TH), granulosa cells (GC) and cumulus cells (CC). Total mRNA was then reverse-transcribed and real-time RT-PCR was performed. The geometric mean of two housekeeping genes (RPL19- ribosomal protein L19 and RPS9- ribosomal protein S9) was used to normalize gene expression. Results of 2 to 4 independent samples are presented as means ± SEM. Bars with different superscripts are significantly different (p < 0.05). (TIF 59 kb)