%0 Online Multimedia %A Greenberg, Jared %A Hrusch, Cara %A Jaffery, Mohammad %A David, Michael %A Daum, Robert %A Hall, Jesse %A Kress, John %A Sperling, Anne %A Verhoef, Philip %D 2018 %T Additional file 3: of Distinct T-helper cell responses to Staphylococcus aureus bacteremia reflect immunologic comorbidities and correlate with mortality %U https://springernature.figshare.com/articles/presentation/Additional_file_3_of_Distinct_T-helper_cell_responses_to_Staphylococcus_aureus_bacteremia_reflect_immunologic_comorbidities_and_correlate_with_mortality/6188108 %R 10.6084/m9.figshare.6188108.v1 %2 https://springernature.figshare.com/ndownloader/files/11207921 %K Sepsis %K Staphylococcus aureus %K Helper T cells %X Figure S2. Method used to classify T cells and CD3+CD4+ cells. CD4+ T cells were first separated into CD25+CD127− (Tregs) or conventional T cells (Tconv). The Tconv were further characterized by expression of CD45RO as a marker of effector/memory T cells. The CD45RO+ cells were then divided into Th1, Th2, or Th17 cells based on the expression of CCR4 and CCR6, with Th1 identified as CCR4−CCR6−, Th2 identified as CCR4+CCR6−, and Th17 identified as CCR6+. (PPTX 84 kb) %I figshare