10.6084/m9.figshare.6164669.v1 Hoi Chu Hoi Chu Kathleen Sprouffske Kathleen Sprouffske Andreas Wagner Andreas Wagner Additional file 4: of Assessing the benefits of horizontal gene transfer by laboratory evolution and genome sequencing Springer Nature 2018 Horizontal gene transfer Experimental evolution Novel nutrient adaptation 2018-04-19 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_4_of_Assessing_the_benefits_of_horizontal_gene_transfer_by_laboratory_evolution_and_genome_sequencing/6164669 Figure S4. The amounts of B, K and W donors in mixed donor-recipient culture decrease rapidly over time. Upper panels: The vertical axes (note the logarithmic scale) show the daily rate of decrease (cfu/ml day; colony forming units per ml of culture per day) in donor density during four consecutive daily serial dilutions, estimated via linear regression [102]. Each colored circle corresponds to data from a donor strain (see color legend), in a mixed culture with either a K recipient and different concentrations of HPA and glycerol (left upper panel), or with a W recipient in different concentrations of butyric acid and glycerol (right upper panel). Lower panels show the nutrient concentrations used (see also Additional file 27: Table S3). We carried out two replicate experiment for each donor-recipient combination per nutrient condition. (EPS 128 kb)