10.6084/m9.figshare.6131783.v1 Jia-Shiun Leu Jia-Shiun Leu So-Yi Chang So-Yi Chang Chia-Yu Mu Chia-Yu Mu Mei-Ling Chen Mei-Ling Chen Bo-Shiun Yan Bo-Shiun Yan Additional file 2: of Functional domains of SP110 that modulate its transcriptional regulatory function and cellular translocation Springer Nature 2018 Functional domain Transcriptional regulation Cellular translocation Nucleolar localization signal Nuclear localization signal 2018-04-11 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Functional_domains_of_SP110_that_modulate_its_transcriptional_regulatory_function_and_cellular_translocation/6131783 Figure S1. Subcellular localization of FLAG-tagged wild-type SP110 or SP110 mutant proteins. (a) Schematic representation of FLAG-tagged proteins containing SP110 deletion mutants. SP100: SP100 domain; SAND: SAND domain; PHD: PHD finger; Bromo: Bromodomain. (b-c) HEK293T cells were transfected with the indicated constructs, and the cellular distribution of FLAG-tagged SP110 proteins (wild-type (b) and mutated forms (c)) was visualized using confocal microscopy at 2 days post-transfection (upper panels). The cells were also subjected to Hoechst staining to identify nuclei (lower panels). Scale bars: 10 μm. The data represent 3 independent experiments. (PDF 738 kb)