10.6084/m9.figshare.6119297.v1 Varsha Pursani Varsha Pursani Deepa Bhartiya Deepa Bhartiya Vivek Tanavde Vivek Tanavde Mohsin Bashir Mohsin Bashir Prabha Sampath Prabha Sampath Additional file 2: of Transcriptional activator DOT1L putatively regulates human embryonic stem cell differentiation into the cardiac lineage Springer Nature 2018 Human embryonic stem cells Cardiac differentiation DOT1L Epigenetics Gene expression Histone methyltransferase 2018-04-10 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Transcriptional_activator_DOT1L_putatively_regulates_human_embryonic_stem_cell_differentiation_into_the_cardiac_lineage/6119297 Characterization of cardiac differentiation of HES3 cells by quantitative real-time PCR (qRT-PCR). Expression of transcripts representing pluripotency (OCT4), cardiac mesoderm (MESP1), cardiac progenitors (NKX2.5, MEF2C), and cardiomyocytes (CTNT) at days 0, 12, and 20 during 20 days of cardiac differentiation. Note OCT-4 expression in undifferentiated cells is downregulated as the cells initiate differentiation. Early cardiac markers detected on day 12 and mature markers upregulated on day 20. Similar changes in transcripts expression observed when KIND1 cells were differentiated into cardiac cells as described earlier [43]. Error bars represent ÂąSEM. (PDF 410 kb)