10.6084/m9.figshare.6119297.v1
Varsha Pursani
Varsha
Pursani
Deepa Bhartiya
Deepa
Bhartiya
Vivek Tanavde
Vivek
Tanavde
Mohsin Bashir
Mohsin
Bashir
Prabha Sampath
Prabha
Sampath
Additional file 2: of Transcriptional activator DOT1L putatively regulates human embryonic stem cell differentiation into the cardiac lineage
Springer Nature
2018
Human embryonic stem cells
Cardiac differentiation
DOT1L
Epigenetics
Gene expression
Histone methyltransferase
2018-04-10 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Transcriptional_activator_DOT1L_putatively_regulates_human_embryonic_stem_cell_differentiation_into_the_cardiac_lineage/6119297
Characterization of cardiac differentiation of HES3 cells by quantitative real-time PCR (qRT-PCR). Expression of transcripts representing pluripotency (OCT4), cardiac mesoderm (MESP1), cardiac progenitors (NKX2.5, MEF2C), and cardiomyocytes (CTNT) at days 0, 12, and 20 during 20 days of cardiac differentiation. Note OCT-4 expression in undifferentiated cells is downregulated as the cells initiate differentiation. Early cardiac markers detected on day 12 and mature markers upregulated on day 20. Similar changes in transcripts expression observed when KIND1 cells were differentiated into cardiac cells as described earlier [43]. Error bars represent ÂąSEM. (PDF 410 kb)