Additional file 5: of Microbiome–host systems interactions: protective effects of propionate upon the blood–brain barrier Lesley Hoyles Tom Snelling Umm-Kulthum Umlai Jeremy Nicholson Simon Carding Robert Glen Simon McArthur 10.6084/m9.figshare.6015020.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_5_of_Microbiome_host_systems_interactions_protective_effects_of_propionate_upon_the_blood_brain_barrier/6015020 Figure S3. Effects of propionate upon expression and activity of typical cerebromicrovascular efflux transporter systems. (a) Surface expression of BCRP, LRP-1 and P-glycoprotein on control and propionate-treated (1 μM, 24 h) hCMEC/D3 cells (black, control, red, propionate), data are representative of three independent experiments. (b) Median fluorescence intensity of surface expression of BCRP, LRP-1 and P-glycoprotein on control and propionate-treated (1 μM, 24 h) hCMEC/D3 cells; data are mean ± SEM, n = 3 independent experiments. (c) Lack of stimulatory effect of propionate upon BCRP, data are mean ± SEM, n = 4. (d) Lack of inhibitory effect of propionate upon stimulated ATP-dependent activity of BCRP, data are mean ± SEM, n = 4. (e) Lack of stimulatory effect of propionate upon P-glycoprotein, data are mean ± SEM, n = 4. (f) Lack of inhibitory effect of propionate upon stimulated ATP-dependent activity of P-glycoprotein, data are mean ± SEM, n = 4. (PDF 437 kb) 2018-03-21 05:00:00 P-glycoprotein propionate PDF 437  kb cerebromicrovascular efflux transporter systems 24  h ATP-dependent activity BCRP data Lack LRP Median fluorescence intensity expression