%0 Journal Article %A Tang, Ruhang %A Jing, Liufang %A Willard, Vincent %A Wu, Chia-lung %A Guilak, Farshid %A Chen, Jun %A Setton, Lori %D 2018 %T Additional file 2: of Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_of_Differentiation_of_human_induced_pluripotent_stem_cells_into_nucleus_pulposus-like_cells/5970163 %R 10.6084/m9.figshare.5970163.v1 %2 https://springernature.figshare.com/ndownloader/files/10704127 %K Human induced pluripotent stem cells %K Intervertebral disc %K Degenerative disc disease %K Nucleus pulposus %K Directed differentiation %K Chondrocyte %K Cartilage %X Figure S1. Showing immunofluorescence of sorted T-GFP+ cells promoted to differentiate in culture. (A) Immunocytofluorescence showed the positive expression of CD24 (green) and (B) positive expression of CD239 (red) in sorted T-GFP+ cells when differentiated in monolayer for 12 days using step 1b and step 2 of NPDM protocol. (C) Yellow denotes colocalization of the markers. All cells counterstained with DAPI. Scale bar = 20 Îźm. (DOCX 995 kb) %I figshare