Additional file 5: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells Severin Mühleder Karoline Pill Mira Schaupper Krystyna Labuda Eleni Priglinger Pablo Hofbauer Verena Charwat Uwe Marx Heinz Redl Wolfgang Holnthoner 10.6084/m9.figshare.5881831.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_5_of_The_role_of_fibrinolysis_inhibition_in_engineered_vascular_networks_derived_from_endothelial_cells_and_adipose-derived_stem_cells/5881831 Thrombin transiently activates EC. HUVEC-ASC clots were fixed and immunofluorescence was performed against E-Selectin after 4 h, 1 day, 4 days and 7 days. Staining revealed transient activation of EC via thrombin after 4 h. TNF-α (10 ng/ml), serving as a positive control, activated EC more strongly after 4 h than thrombin alone. No aprotinin was used in any sample. n = 8 from two independent experiments. Scale bar: 200 μm. (DOC 322 kb) 2018-02-12 05:00:00 Vascularisation Fibrin Endothelial cells Adipose-derived stem cells