Peng, Ran Wang, Ye Feng, Wan-wan Yue, Xin-jing Chen, Jiang-he Hu, Xiao-zhuang Li, Zhi-feng Sheng, Duo-hong Zhang, You-ming Li, Yue-zhong MOESM4 of CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus Additional file 4: Figure S4. The sequence and expression vector of sgRNA. The promoter of pilA gene (shown in orange color) from M. xanthus drives transcription of sgRNAs. The sgRNA fragment containing the 20-nt specific targeting sequence (green), the tracrRNA scaffold (blue) and the human U6 terminator (red). CRISPR/dCas9 activation;sgRNAs;Activator proteins;Promoter;Transcriptional improvement;Biosynthetic gene cluster;Epothilones;Myxococcus xanthus 2018-01-29
    https://springernature.figshare.com/articles/figure/MOESM4_of_CRISPR_dCas9-mediated_transcriptional_improvement_of_the_biosynthetic_gene_cluster_for_the_epothilone_production_in_Myxococcus_xanthus/5834502
10.6084/m9.figshare.5834502.v1