MOESM4 of CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus
Ran Peng
Ye Wang
Wan-wan Feng
Xin-jing Yue
Jiang-he Chen
Xiao-zhuang Hu
Zhi-feng Li
Duo-hong Sheng
You-ming Zhang
Yue-zhong Li
10.6084/m9.figshare.5834502.v1
https://springernature.figshare.com/articles/figure/MOESM4_of_CRISPR_dCas9-mediated_transcriptional_improvement_of_the_biosynthetic_gene_cluster_for_the_epothilone_production_in_Myxococcus_xanthus/5834502
Additional file 4: Figure S4. The sequence and expression vector of sgRNA. The promoter of pilA gene (shown in orange color) from M. xanthus drives transcription of sgRNAs. The sgRNA fragment containing the 20-nt specific targeting sequence (green), the tracrRNA scaffold (blue) and the human U6 terminator (red).
2018-01-29 05:00:00
CRISPR/dCas9 activation
sgRNAs
Activator proteins
Promoter
Transcriptional improvement
Biosynthetic gene cluster
Epothilones
Myxococcus xanthus