MacConaill, Laura Burns, Robert Nag, Anwesha Coleman, Haley Slevin, Michael Giorda, Kristina Light, Madelyn Lai, Kevin Jarosz, Mirna McNeill, Matthew Ducar, Matthew Meyerson, Matthew Thorner, Aaron Additional file 3: Figure S3. of Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing Schematic of the sources of sample cross-talk including adapter contamination, index hopping during multiplex target enrichment post-capture PCR, index hopping during cluster amplification, and demultiplexing errors. Unique dual-matched indices reduce read misassignment because unexpected index combinations are removed from downstream analysis. (PDF 936 kb) Next generation sequencing;Massively parallel sequencing;Adapter;Index;Barcode cross-talk;Illumina;Multiplexing;UMI;Molecular barcode 2018-01-08
    https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_Figure_S3_of_Unique_dual-indexed_sequencing_adapters_with_UMIs_effectively_eliminate_index_cross-talk_and_significantly_improve_sensitivity_of_massively_parallel_sequencing/5768856
10.6084/m9.figshare.5768856.v1