Additional file 2: Figure S1. of Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method Heidi Hongisto Tanja Ilmarinen Meri Vattulainen Alexandra Mikhailova Heli Skottman 10.6084/m9.figshare.5742387.v1 https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S1_of_Xeno-_and_feeder-free_differentiation_of_human_pluripotent_stem_cells_to_two_distinct_ocular_epithelial_cell_types_using_simple_modifications_of_one_method/5742387 Human iPSC line hiPSC1 cultured on LN-521 in E8 medium maintained pluripotent characteristics. A) Typical undifferentiated morphology and B) expression of pluripotency markers NANOG, OCT-3/4, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81, as well as corresponding nuclear stains with DAPI after 4 passages in feeder-free culture. C) Pluripotency shown as expression of markers of the three embryonic germ layers, namely SOX17 for endoderm, OTX2 for ectoderm, and SMA for mesoderm after spontaneous differentiation (feeder-free passage level 4). All scale bars = 200 μm. D) Cells showing normal female karyotype after nine passages. (TIF 5463 kb) 2017-12-29 05:00:00 Human embryonic stem cells Human induced pluripotent stem cells Retinal pigment epithelial cells Corneal limbal epithelial stem cells Xeno-free Feeder-free