10.6084/m9.figshare.5726437.v1 Rosemary A. Bamford Rosemary A. Bamford Ashley Smith Ashley Smith Jeremy Metz Jeremy Metz Georgina Glover Georgina Glover Richard W. Titball Richard W. Titball Stefano Pagliara Stefano Pagliara Additional file 2: Figure S2. of Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy Springer Nature 2017 Viable but non-culturable cells Microfluidics Escherichia coli Persisters Single-cell Antibiotic tolerance Non-growing cells 2017-12-21 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_Investigating_the_physiology_of_viable_but_non-culturable_bacteria_by_microfluidics_and_time-lapse_microscopy/5726437 Bulk and single-cell persister enumeration. Dependence of the frequency of persister cells on ampicillin concentration as measured via the single-cell microfluidics-microscopy assay (full circles) illustrated in Fig. 1 and the colony forming unit assay (open circles). The two assays are performed on aliquots withdrawn from the same E. coli overnight culture. Data and error bars are mean and standard error of the mean of measurements obtained in biological triplicate (N = 3). Data agreement within experimental error confirms the validity of the newly developed microfluidic assay. (PNG 226 kb)