MOESM4 of Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells Yiyang Qin Ikuo Sekine Mengmeng Fan Yuichi Takiguchi Yuji Tada Masato Shingyoji Michiko Hanazono Naoto Yamaguchi Masatoshi Tagawa 10.6084/m9.figshare.5691862.v1 https://springernature.figshare.com/articles/journal_contribution/MOESM4_of_Augmented_expression_of_cardiac_ankyrin_repeat_protein_is_induced_by_pemetrexed_and_a_possible_marker_for_the_pemetrexed_resistance_in_mesothelioma_cells/5691862 Additional file 4: Figure S3. Inhibited secretion of IGFBP3 with si-RNA. H226-PEM cells were transfected with si-RNA for IGFBP3 (20 nM) (s7227, s7228 and s7229) or control si-RNA (si-Cont), and the culture supernatants were assayed with ELISA. We measured optical density at 450 nm and used 10 ng rhIGFBP3 as a positive control. Average and SE bars are shown (n=3). 2017-12-11 05:00:00 Mesothelioma PEM resistance Cardiac ankyrin repeat protein Biomarker