10.6084/m9.figshare.5691862.v1 Yiyang Qin Yiyang Qin Ikuo Sekine Ikuo Sekine Mengmeng Fan Mengmeng Fan Yuichi Takiguchi Yuichi Takiguchi Yuji Tada Yuji Tada Masato Shingyoji Masato Shingyoji Michiko Hanazono Michiko Hanazono Naoto Yamaguchi Naoto Yamaguchi Masatoshi Tagawa Masatoshi Tagawa MOESM4 of Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells Springer Nature 2017 Mesothelioma PEM resistance Cardiac ankyrin repeat protein Biomarker 2017-12-11 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM4_of_Augmented_expression_of_cardiac_ankyrin_repeat_protein_is_induced_by_pemetrexed_and_a_possible_marker_for_the_pemetrexed_resistance_in_mesothelioma_cells/5691862 Additional file 4: Figure S3. Inhibited secretion of IGFBP3 with si-RNA. H226-PEM cells were transfected with si-RNA for IGFBP3 (20 nM) (s7227, s7228 and s7229) or control si-RNA (si-Cont), and the culture supernatants were assayed with ELISA. We measured optical density at 450 nm and used 10 ng rhIGFBP3 as a positive control. Average and SE bars are shown (n=3).