10.6084/m9.figshare.5683087.v1 Charlotte Vanmarsenille Charlotte Vanmarsenille Inés Díaz del Olmo Inés Díaz del Olmo Jelle Elseviers Jelle Elseviers Gholamreza Hassanzadeh Ghassabeh Gholamreza Hassanzadeh Ghassabeh Kristof Moonens Kristof Moonens Didier Vertommen Didier Vertommen An Martel An Martel Freddy Haesebrouck Freddy Haesebrouck Frank Pasmans Frank Pasmans Jean-Pierre Hernalsteens Jean-Pierre Hernalsteens Henri De Greve Henri De Greve MOESM6 of Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization Springer Nature 2017 L 6 membrane protein PCR GAATTTGTAAAGAGCTTGAAG coli porA gene acid sequences Nanobodie ATGAAACTAGTTAAACTTAGTTTA MOESM 6 MOMP structure Loop β- strands tool al 6. Alignment Ferrara reference strain primers Campylobacter colonization region jejuni KC 40 L 1 loop MOMP-encoding porA L 7 epitope 2017-12-08 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM6_of_Nanobodies_targeting_conserved_epitopes_on_the_major_outer_membrane_protein_of_Campylobacter_as_potential_tools_for_control_of_Campylobacter_colonization/5683087 Additional file 6. Alignment of amino acid sequences of the MOMP-encoding porA -gene. The porA gene of the C. jejuni and C. coli isolates (Table 1) was amplified using PCR and aligned, using C. jejuni KC40 as a reference strain, to identify conserved regions. The PCR was performed with the primers F3 (5′-ATGAAACTAGTTAAACTTAGTTTA-3′) and R3 (5′-GAATTTGTAAAGAGCTTGAAG-3′). External loops are labelled from L1 to L7 and β-strands are underlined, based on the MOMP structure determined by Ferrara et al. [39]. Loops L3 and L6 are the most conserved in sequence and number of amino acids.