10.6084/m9.figshare.5683087.v1
Charlotte Vanmarsenille
Charlotte
Vanmarsenille
Inés Díaz del Olmo
Inés
Díaz del Olmo
Jelle Elseviers
Jelle
Elseviers
Gholamreza Hassanzadeh Ghassabeh
Gholamreza
Hassanzadeh Ghassabeh
Kristof Moonens
Kristof
Moonens
Didier Vertommen
Didier
Vertommen
An Martel
An
Martel
Freddy Haesebrouck
Freddy
Haesebrouck
Frank Pasmans
Frank
Pasmans
Jean-Pierre Hernalsteens
Jean-Pierre
Hernalsteens
Henri De Greve
Henri
De Greve
MOESM6 of Nanobodies targeting conserved epitopes on the major outer membrane protein of Campylobacter as potential tools for control of Campylobacter colonization
Springer Nature
2017
L 6
membrane protein
PCR
GAATTTGTAAAGAGCTTGAAG
coli
porA gene
acid sequences
Nanobodie
ATGAAACTAGTTAAACTTAGTTTA
MOESM 6
MOMP structure
Loop
β- strands
tool
al
6. Alignment
Ferrara
reference strain
primers
Campylobacter colonization
region
jejuni KC 40
L 1
loop
MOMP-encoding porA
L 7
epitope
2017-12-08 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/MOESM6_of_Nanobodies_targeting_conserved_epitopes_on_the_major_outer_membrane_protein_of_Campylobacter_as_potential_tools_for_control_of_Campylobacter_colonization/5683087
Additional file 6. Alignment of amino acid sequences of the MOMP-encoding porA -gene. The porA gene of the C. jejuni and C. coli isolates (Table 1) was amplified using PCR and aligned, using C. jejuni KC40 as a reference strain, to identify conserved regions. The PCR was performed with the primers F3 (5′-ATGAAACTAGTTAAACTTAGTTTA-3′) and R3 (5′-GAATTTGTAAAGAGCTTGAAG-3′). External loops are labelled from L1 to L7 and β-strands are underlined, based on the MOMP structure determined by Ferrara et al. [39]. Loops L3 and L6 are the most conserved in sequence and number of amino acids.