10.6084/m9.figshare.5648608.v1 Daun Jeon Daun Jeon Seok Joong Kim Seok Joong Kim Hong Seok Kim Hong Seok Kim Additional file 1: Figure S1. of Anti-inflammatory evaluation of the methanolic extract of Taraxacum officinale in LPS-stimulated human umbilical vein endothelial cells Springer Nature 2017 Taraxacum officinale Anti-inflammation Endothelial cell Endotoxin NF-κb 2017-11-29 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_Anti-inflammatory_evaluation_of_the_methanolic_extract_of_Taraxacum_officinale_in_LPS-stimulated_human_umbilical_vein_endothelial_cells/5648608 Comparison of TO efficacy with acetylsalicylic acid (ASA). HUVECs were pretreated with TO (100 μg/ml) or different concentrations of ASA for one hour, and then stimulated with 1 μg/ml LPS for 24 h. VCAM-1 and actin levels were assessed by Western blot analysis. Arrow head indicates VCAM-1. Figure S2. TO decreases VCAM-1 induction dose dependently but does not affect ICAM-1 levels in LPS-stimulated HUVECs. HUVECs were pretreated with different concentrations of TO for one hour, and then stimulated with 1 μg/ml LPS for 24 h. VCAM-1, ICAM-1, and actin levels were assessed by Western blot analysis. Arrow head indicates VCAM-1. Figure S3. TO does not affect MAPK activation induced by LPS stimulation in HUVECs. HUVECs were preincubated with TO (100 μg/ml) for one hour and then stimulated with LPS (1 μg/ml) for 30 min. MAPKs activation was assessed by Western blot analysis. (PDF 368 kb)