Additional file 1: of Effects of urban coarse particles inhalation on oxidative and inflammatory parameters in the mouse lung and colon Cécile Vignal Muriel Pichavant Laurent Alleman Madjid Djouina Florian Dingreville Esperanza Perdrix Christophe Waxin Adil Ouali Alami Corinne Gower-Rousseau Pierre Desreumaux Mathilde Body-Malapel 10.6084/m9.figshare.c.3937711_D1.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Effects_of_urban_coarse_particles_inhalation_on_oxidative_and_inflammatory_parameters_in_the_mouse_lung_and_colon/5627869 Figure S1. Location of the sampling site. Figure S2. Meteorological conditions during the sampling period. A Temperature. B Relative humidity. C Rain. D Wind speed. E Wind direction and frequency. Figure S3. Comparison of trace element concentrations in cPMD collected at Douai and coarse PM collected in Helsinki, Finland [30] and Budapest, Hungary [31]. Figure S4. Effects of NAC in the basal condition. All mice inhaled sterile water. Mice that received NAC (15 μg/kg/day for 14 days) in drinking water were compared with CT mice that did not receive NAC in drinking water. A Serum MDA levels. B iNKT cell count measured by flow cytometry. C Quantitative PCR (qPCR) analysis of cytokine and chemokine mRNA levels in the lung. D qPCR analysis of mRNA levels of oxidative stress markers in the lung. E MPO activity in the colon. F qPCR analysis of cytokine and chemokine mRNA levels in the colon. Data are presented as mean ± SEM. *p < 0.05, Mann-Whitney U test. (DOCX 1064 kb) 2017-11-22 05:00:00 Particulate matter Coarse PM Oxidative stress Inflammation Gut-lung axis N-acetyl-L-cysteine