Oesterle, Sabine Roberts, Tania Widmer, Lukas Mustafa, Harun Panke, Sven Billerbeck, Sonja Additional file 1: Table S1. of Sequence-based prediction of permissive stretches for internal protein tagging and knockdown Mapping of known permissive sites within TpiA and Bla. A known permissive site (first column, given as residue number) within TpiA and Bla [32] was assigned to a predicted stretch (PS) if it mapped directly within a PS (see Additional file 3: Figure S1 for illustration) or if it was shifted by few residues but at least mapped to the same secondary structural element (“scattered” permissive sites). The number of residues by which a given “scattered” site is shifted is given in column 5. Note that, due to the experimental approach, which selected for only functional protein variants, there are no known non-permissive sites for TpiA. (DOCX 90 kb) Permissive site;Internal protein tagging;TEV protease;Protein knockdowns;Cell-free biotechnology 2017-10-30
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