10.6084/m9.figshare.c.3916024_D3.v1 Tohnyui Ndinyanka Fabrice Tohnyui Ndinyanka Fabrice Andres Kaech Andres Kaech Gery Barmettler Gery Barmettler Christof Eichenberger Christof Eichenberger J. Paul Knox J. Paul Knox Ueli Grossniklaus Ueli Grossniklaus Christoph Ringli Christoph Ringli Additional file 3: of Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation Springer Nature 2017 Arabidopsis Callose Cell wall Electron microscopy Immunolabeling Pollen Pollen tube Stylar tissue Transmitting tract Ultrastructure 2017-10-27 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Efficient_preparation_of_Arabidopsis_pollen_tubes_for_ultrastructural_analysis_using_chemical_and_cryo-fixation/5548426 Comparison of the ChF, PF, and HPF ultrastructural details of PTs within the pistil. The intracellular ultrastructural details of PTs within the TT achieved by HPF (A), PF (B) and ChF (C). Except for the lipid droplets which are best resolved by ChF, the resolution of most intracellular features is highest in HPF, followed by PF and then ChF sections. Ld = Lipid bodies, g = Golgi, er = endoplasmic reticulum, v = vacuole, m = mitochondria Scale bar: 500 nm. (DOCX 531 kb)