MOESM2 of Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts HuangRuixia AndersenLise RofstadEinar 2017 Additional file 2. Histological preparations of a representative C-10 tumor and a representative T-22 tumor immunostained for pimonidazole to visualize tumor hypoxia, immunostained for CD31 to visualize tumor blood vessels, and immunostained for LYVE-1 to visualize peritumoral lymphatics in skin.