Additional file 1: Figure S1. of Zebrafish knockout of Down syndrome gene, DYRK1A, shows social impairments relevant to autism Oc-Hee Kim Hyun-Ju Cho Enna Han Ted Hong Krishan Ariyasiri Jung-Hwa Choi Kyu-Seok Hwang Yun-Mi Jeong Se-Yeol Yang Kweon Yu Doo-Sang Park Hyun-Woo Oh Erica Davis Charles Schwartz Jeong-Soo Lee Hyung-Goo Kim Cheol-Hee Kim 10.6084/m9.figshare.c.3893251_D2.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_Zebrafish_knockout_of_Down_syndrome_gene_DYRK1A_shows_social_impairments_relevant_to_autism/5457790 Characterization of early neural development and larval behavioral tests in WT and dyrk1aa KO fish. (A-F) Whole-mount in situ hybridization analysis with various molecular markers at 24 hpf (A, B, lateral view) and 48 hpf (C-F, rostral dorsal view): sox2, neural stem cell marker; neurog1, neuronal determination marker; and cyclin D1, cell proliferation marker. Anterior is to the left. Number of fish used for the analysis: 1) after sox2 staining and photography, each embryo was genotyped for WT (5/19) and KO homozygote (3/19); 2) for neurog1, it was WT (6/16) and KO homozygote (4/16); and 3) for cyclin D1, WT (3/16) and KO homozygote (5/16), respectively. (G) Locomotion response to dark flashes in WT and dyrk1aa KO larvae at 6 dpf. Movement distance was measured by video tracking analysis (cm per every 10 s). With light-on in 30 s-intervals, zebrafish larvae showed a freezing response (yellow box in the graph). However, they showed a startle response to light-off dark condition (gray box). The number of fish used for this assay: n = 14 for WT (+/+), n = 25 for heterozygote (+/−), and n = 9 for KO homozygote (−/−). (H) Circadian rhythms in WT and dyrk1aa KO larvae between 5 and 7 dpf. Circadian rhythms of locomotor activity under LD (day-night) cycles were measured. Both WT and dyrk1aa KO larvae display a similar pattern of locomotor activity in daytime or nighttime. The number of fish used for this assay: n = 11 for control heterozygote (+/−), and n = 13 for KO homozygote (−/−). Data are presented as mean ± SEM. (PDF 1219 kb) 2017-09-29 05:00:00 Autism Down syndrome Knockout Zebrafish DYRK1A Social interaction Shoaling Group behavior