10.6084/m9.figshare.c.3893251_D2.v1
Oc-Hee Kim
Oc-Hee
Kim
Hyun-Ju Cho
Hyun-Ju
Cho
Enna Han
Enna
Han
Ted Hong
Ted
Hong
Krishan Ariyasiri
Krishan
Ariyasiri
Jung-Hwa Choi
Jung-Hwa
Choi
Kyu-Seok Hwang
Kyu-Seok
Hwang
Yun-Mi Jeong
Yun-Mi
Jeong
Se-Yeol Yang
Se-Yeol
Yang
Kweon Yu
Kweon
Yu
Doo-Sang Park
Doo-Sang
Park
Hyun-Woo Oh
Hyun-Woo
Oh
Erica Davis
Erica
Davis
Charles Schwartz
Charles
Schwartz
Jeong-Soo Lee
Jeong-Soo
Lee
Hyung-Goo Kim
Hyung-Goo
Kim
Cheol-Hee Kim
Cheol-Hee
Kim
Additional file 1: Figure S1. of Zebrafish knockout of Down syndrome gene, DYRK1A, shows social impairments relevant to autism
Springer Nature
2017
Autism
Down syndrome
Knockout
Zebrafish
DYRK1A
Social interaction
Shoaling
Group behavior
2017-09-29 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_Zebrafish_knockout_of_Down_syndrome_gene_DYRK1A_shows_social_impairments_relevant_to_autism/5457790
Characterization of early neural development and larval behavioral tests in WT and dyrk1aa KO fish. (A-F) Whole-mount in situ hybridization analysis with various molecular markers at 24 hpf (A, B, lateral view) and 48 hpf (C-F, rostral dorsal view): sox2, neural stem cell marker; neurog1, neuronal determination marker; and cyclin D1, cell proliferation marker. Anterior is to the left. Number of fish used for the analysis: 1) after sox2 staining and photography, each embryo was genotyped for WT (5/19) and KO homozygote (3/19); 2) for neurog1, it was WT (6/16) and KO homozygote (4/16); and 3) for cyclin D1, WT (3/16) and KO homozygote (5/16), respectively. (G) Locomotion response to dark flashes in WT and dyrk1aa KO larvae at 6 dpf. Movement distance was measured by video tracking analysis (cm per every 10 s). With light-on in 30 s-intervals, zebrafish larvae showed a freezing response (yellow box in the graph). However, they showed a startle response to light-off dark condition (gray box). The number of fish used for this assay: n = 14 for WT (+/+), n = 25 for heterozygote (+/−), and n = 9 for KO homozygote (−/−). (H) Circadian rhythms in WT and dyrk1aa KO larvae between 5 and 7 dpf. Circadian rhythms of locomotor activity under LD (day-night) cycles were measured. Both WT and dyrk1aa KO larvae display a similar pattern of locomotor activity in daytime or nighttime. The number of fish used for this assay: n = 11 for control heterozygote (+/−), and n = 13 for KO homozygote (−/−). Data are presented as mean ± SEM. (PDF 1219 kb)