MOESM6 of Identification of two mutation sites in spike and envelope proteins mediating optimal cellular infection of porcine epidemic diarrhea virus from different pathways Min Sun Jiale Ma Zeyanqiu Yu Zihao Pan Chengping Lu Huochun Yao 10.6084/m9.figshare.c.3867826_D6.v1 https://springernature.figshare.com/articles/figure/MOESM6_of_Identification_of_two_mutation_sites_in_spike_and_envelope_proteins_mediating_optimal_cellular_infection_of_porcine_epidemic_diarrhea_virus_from_different_pathways/5363065 Additional file 6. The dual-staining IFA assay to verify the overexpression of PEDV parent E protein and mutant E proteins in Vero cells. The recombinant plasmids (PI-E or PI-mE) were transfected into Vero cells for 24 h. The corresponding amount of empty plasmid (pIRES2-EGFP) was used as the mock control. The primary antibody was the anti-HA-tag antibody. The red staining (recombinant protein) was almost merged with the green staining (EGFP-tag protein). 2017-08-30 05:00:00 antibody PI-E dual-staining IFA assay Vero cells PEDV parent E protein porcine epidemic diarrhea virus 24 2-EGFP MOESM plasmid