MOESM6 of Identification of two mutation sites in spike and envelope proteins mediating optimal cellular infection of porcine epidemic diarrhea virus from different pathways
Min Sun
Jiale Ma
Zeyanqiu Yu
Zihao Pan
Chengping Lu
Huochun Yao
10.6084/m9.figshare.c.3867826_D6.v1
https://springernature.figshare.com/articles/figure/MOESM6_of_Identification_of_two_mutation_sites_in_spike_and_envelope_proteins_mediating_optimal_cellular_infection_of_porcine_epidemic_diarrhea_virus_from_different_pathways/5363065
Additional file 6. The dual-staining IFA assay to verify the overexpression of PEDV parent E protein and mutant E proteins in Vero cells. The recombinant plasmids (PI-E or PI-mE) were transfected into Vero cells for 24Â h. The corresponding amount of empty plasmid (pIRES2-EGFP) was used as the mock control. The primary antibody was the anti-HA-tag antibody. The red staining (recombinant protein) was almost merged with the green staining (EGFP-tag protein).
2017-08-30 05:00:00
antibody
PI-E
dual-staining IFA assay
Vero cells
PEDV parent E protein
porcine epidemic diarrhea virus
24Â
2-EGFP
MOESM
plasmid