Additional file 3: of Comprehensive comparison of three different animal models for systemic inflammation SeemannSemjon ZohlesFranziska LuppAmelie 2017 iNOS expression in the livers and spleens as well as HE staining in the spleens of PCI-treated mice. At the time point indicated, mice were sacrificed and livers and spleens were collected for immunohistochemical analysis. (a) Course of iNOS expression (red-brown color, counterstaining with hematoxylin; original magnification: 400×) in the livers of PCI-treated mice as supplements to Fig. 4a and b. Arrows exemplarily show the infiltrating neutrophils. (b) HE-stained spleens 24 h after PCI treatment (original magnification: 400×). (c) iNOS expression patterns after PCI treatment at 24 h (original magnification: 400×) as a supplemental to Fig. 6e. (TIFF 1827 kb)