Robert, Jérôme Button, Emily Stukas, Sophie Boyce, Guilaine Gibbs, Ebrima Cowan, Catherine Gilmour, Megan Cheng, Wai Soo, Sonja Yuen, Brian Bahrabadi, Arvin Kang, Kevin Kulic, Iva Francis, Gordon Cashman, Neil Wellington, Cheryl Additional file 6: of High-density lipoproteins suppress Aβ-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture Adhesion molecules are enhanced by TNF-α but not Aβ.(a-c) hCMEC/D3 were primed with 100 μg/mL HDL for 2 h followed by stimulation with 1 ng/mL TNF-α for 3 h. Cell lysates were prepared in RIPA and protein levels of (a) ICAM-1 and (b) VCAM-1 were measured by denaturing immunoblotting (c). (d-g) hCMEC/D3 were stimulated with monomeric (d,f) Aβ40 or (e,g) Aβ42 at the indicated concentrations and (d,e) ICAM-1 and (f,g) VCAM-1 mRNA levels were measured by real-time PCR. (h-j) Following Aβ stimulation, cell surface proteins were biotinylated and isolated by immunoprecipitation. Protein levels of cell surface and total (h) ICAM-1 and (i) VCAM-1 were measured by denaturing immunoblotting (j). (k-m) hCMEC/D3 were pre-treated with 100 μg/mL of HDL for 2 h followed by stimulation with Aβ. After 3 h total and cell surface ICAM-1 expression were measured as above. Graphs represent means ± SD from at least 3 independent trials. ***p < 0.001 versus vehicle, § p < 0.05, versus Aβ or TNF-α (PDF 2282 kb) Alzheimer’s disease;Beta-amyloid;High-density lipoprotein;Engineered vessel;Endothelial cells 2017-08-22
    https://springernature.figshare.com/articles/journal_contribution/Additional_file_6_of_High-density_lipoproteins_suppress_A_-induced_PBMC_adhesion_to_human_endothelial_cells_in_bioengineered_vessels_and_in_monoculture/5335531
10.6084/m9.figshare.c.3860566_D6.v1